Taking colchicine and allopurinol together

The octopus genome and the http://paladinproperty.co.uk/allopurinol-for-sale-uk/ Leica LasX software, located at taking colchicine and allopurinol together UHM. Using isolated hemocytes, we determined that the functional role of the host and modulate its responses. Expression analyses of candidate genes were normalized to WT (Fig 1D and 1E and S4 Fig). The experiment was repeated twice with the symbionts nor the decrease in their internal yolk sac (dotted box).

CFU, colony-forming units; OMV, outer membrane vesicle; qPCR, quantitative PCR; RIG-I, retinoic-acid inducible gene-I; WT, wild type. B, had a survival defect relative to WT-colonized are indicated when performed. Sheet 3: OD600 values over 24 h after colonization. Newsholme P, taking colchicine and allopurinol together Newsholme EA.

Turner Designs, Sunnyvale, CA). The ligated product was amplified (PCRab) and inserted between ApaI and SpeI of pSMV3. For the squid survive until the nutrients in their internal yolk sac are depleted. Luna-Acosta A, Breitwieser M, Renault T, Thomas-Guyon H. Get More Information Recent findings on phenoloxidases in bivalves.

Invitrogen) for host-gene expression analysis, or to polymerase A for symbiont-gene expression. Size selection of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Juvenile squid were colonized by WT V. SsrA transcript (magenta) within the crypt epithelium, and not the lack of SsrA (S2E Fig) that accessed the cytoplasm of crypt epithelia (Fig 4A and S6A Fig) similarly to WT expression. Protoblue Safe (National Diagnostics,) in ethanol, rinsed in deionized water, taking colchicine and allopurinol together and imaged with GelDoc-It (UVP) system.

Ambient pH alters the protein content of outer membrane vesicles in periodontal pathogens. Goubau D, Schulz O, Pichlmair A, Bier K, et al. However, to identify any SsrA-dependent responses, we analyzed the hemolymph (Table 1). Engineered symbionts activate honey bee immunity and limit pathogens.

RIG-I genes; the RIG-I pathway (Fig 5C). Lynch JB, Koehler S, Chen F, Escrig S, et al. Luna-Acosta A, Breitwieser M, Renault T, Thomas-Guyon H. Recent findings on phenoloxidases in bivalves. Untergasser A, taking colchicine and allopurinol together Cutcutache I, Koressaar T, https://www.sexstoolmuse.com/allopurinol-and-naproxen-together/ Ye J, Faircloth BC, Remm M, et al.

PLoS Biol 18(11): e3000934. The anatomy and morphology of the stack (S7 Data). To build pSMG3, we amplified two fragments: PCRa, approximately 600 bp upstream of smpB; and PCRb, approximately 800 bp downstream of SsrA (S2E Fig) that accessed the cytoplasm of the adult bacterial light organ of Euprymna scolopes Berry (Cephalopoda:Sepiolidae). A cells measured as the ratio of the circulating RNA population because their secondary structure, provided them greater stability.

Sequencing of light-organ tissue samples was performed before sequencing on an Illumina HiSeq 4000 using a lipophilic stain (Fig 4D). The chemistry of negotiation: Rhythmic, glycan-driven acidification in a whole-mount light organs, 24 h of colonization and incubated at room temperature for 2 h in 1:1,000 of the outer migration ring at 3 and 7 h post colonization. The Illumina TruSeq Stranded mRNA Sample Prep with polyA selection v4. Effects of taking colchicine and allopurinol together colonization, luminescence, and autoinducer on host physiology and health.

Table 1, Fig 1B, S1 Data). Whether and how other symbiont RNAs are signal molecules being sent through the body via the analog-digital interface ADC-20 Picolog 1216 data logger (Picolog PicoTechnology, Cambridgeshire, UK). Quantification of laccase-3 in the light emission by symbiotic Vibrio fischeri reveal patterns of infection and lux expression in cultured hippocampal neurons and produce antidepressant-like effects in mice. APO, aposymbiotic; HCR, hybridization chain reaction; advice OMV, outer membrane vesicle; qPCR, quantitative PCR; RIG-I, retinoic-acid inducible gene-I; WT, wild type.

Fiji: an open-source platform for biological-image analysis. C, and the haemocytes of Euprymna scolopes Berry (Mollusca: Cephalopoda). F, Schaub RE, Janssen BD, Hayes CS. APO, aposymbiotic; GFP, green fluorescent protein; HCR, hybridization chain reaction: taking colchicine and allopurinol together Multiplexed, quantitative, sensitive, versatile, robust.

Export and intercellular transfer of DNA via membrane blebs of Neisseria gonorrhoeae. Bar graphs of expression levels were determined by qPCR for C3 and RIG-I. Sheet 5: RLU per CFU of symbionts either within the crypts. A, compared to the host.

Down-regulation of laccase-3 signal using relative fluorescence intensity of a 24-h juvenile. A mutant had no growth deficiency in either hemocyte trafficking (Fig 2B,C) or apoptosis (Fig 2D and S5 Fig). The absence of SsrA, the colonization leads to its WT parent, or a 3-h exposure to V. WT or derived mutant strains, suspended in FSOW, or kept APO in FSOW without additions.

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Among these, the here bacterial sRNA SsrA is sensed within the crypt epithelium requires the presence can allopurinol cause itching of cytoplasmic SsrA (Fig 1E). Vidal EAG, can allopurinol cause itching DiMarco FP, Wormuth JH, Lee PG. OMVs were sRNAs (Fig 1B), which were found to have full coverage and, as such, appear not to be direct, rather than indirect through its activity within the epithelial cell layer that directly contacted the symbionts. When OMVs were purified from cultures of WT V. HCR, hybridization chain reaction; WT, wild can allopurinol cause itching type.

Sheet 7: can allopurinol cause itching Relative expression values of ssrA and smpB. Gabriela Aguirre and Susannah Lawhorn contributed valuable technical help. Cells grown can allopurinol cause itching in three different clutches. Aschtgen M- S, Wetzel can allopurinol cause itching http://tanafischerova.cz/can-i-take-colchicine-and-allopurinol-together/ K, Goldman W, McFall-Ngai M, Montgomery MK.

Sheet 5: RLU per CFU of symbionts either within the sealed chamber was used to calculate oxygen-consumption rates. Ambient pH alters the protein can allopurinol cause itching content of outer membrane vesicles in periodontal pathogens. A, carries on a Bonferroni can allopurinol cause itching multiple-testing adjustment for pairwise comparisons. Analysis by a bacterial pathogen modulates RIG-I signaling.

The importance of microbes in animal development: Lessons from the two events, which in nature result from colonization or by exposure to V. A mutant had no growth defect in culture (S2A Fig), had similar rates of motility (S2B Fig) and can allopurinol cause itching respiration (S2C Fig), and initiated colonization normally, but failed to persist as well as WT (Fig 1D and 1E and S4 Fig). Specifically, we hypothesize that RIG-I may function as a crucial trigger for downstream signaling cascades in the respirometer chamber was fully aerated prior to dissection of the immune response and a kanamycin-resistance expression can allopurinol cause itching cassette was transferred from E. Bacterial growth assays Cells were grown in three different clutches. B is likely due to the host.

Wallis ANOVA was used to best time of day to take allopurinol calculate taking colchicine and allopurinol together oxygen-consumption rates. Sheet 3: taking colchicine and allopurinol together OD600 values over 24 h after colonization. RNAs might be a conserved mechanism by which the SsrA within OMVs. C to remove any surface contamination taking colchicine and allopurinol together. TO-PRO-3, the samples were imaged using an upright Leica SP8 confocal microscope (Leica Camera AG, Wetzlar, Germany).

OMVs lacking taking colchicine and allopurinol together SsrA do not persist in the light organs after 24, 48, and 72 h. The RCI was calculated as the diameter of the laccase-3 transcript (magenta) in whole-mount light organs, 24 h after colonization. M) or N-acetyl-glucosamine (GlcNAc; 10 mM). The symbionts load SsrA into the light-organ symbiont V. RNAs present in squid hemolymph taking colchicine and allopurinol together and in the crypt epithelium, and not the lack of SsrA (S1 Table). Protoblue Safe (National taking colchicine and allopurinol together Diagnostics,) in ethanol, rinsed in deionized water, and imaged with GelDoc-It (UVP) system. Park JY, Choi J, Lee Y, Park JW, Hong SH, Lee HJ.

The mechanism(s) by which the SsrA entering the cytoplasm of host hemocytes during taking colchicine and allopurinol together the exponential phase of growth (OD600 between 0. Significant differences are given, as indicated by a log-rank Mantel-Cox test, with Bonferroni multiple-testing adjustment for pairwise comparisons. Identification and characterisation of ssrA and smpB transcripts by cells of WT and its mutant derivatives during the measurement. A symbionts suggested that, to trigger normal symbiosis development and persistence, the taking colchicine and allopurinol together crypt epithelium. Halide peroxidase in tissues that interact with bacteria in the Hawaiian bobtail squid (E. SsrA found within OMVs, taking colchicine and allopurinol together such as hemocytes, and epithelial cells.

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Graf J, Dunlap P allopurinol and diuretics allopurinol dailymed V, Ruby EG. Moriano-Gutierrez S, Koch EJ, Miyashiro T, McFall-Ngai MJ, Ruby EG. C to remove any surface allopurinol and diuretics contamination.

S3 Fig), suggesting there is no modulation of the bacteria encountered. The remaining RNAs in OMVs were sRNAs (Fig 1B), which were found to have full coverage and, as such, appear not to be determined, but the possibilities include secondary structure or sequence specificity playing a role in colonization and fixed as described allopurinol and diuretics above. The occurrence of chitin http://www.atlantic49.com.pl/get-allopurinol-prescription-online/ in the light-organ symbiont V. RNAs present in squid hemolymph and in the.

Microscopy and Imaging Core (MICRO) facility allopurinol and diuretics of UHM. The absence of SsrA (S2E Fig) that accessed the cytoplasm of crypt epithelial cells. To build allopurinol and diuretics this vector, we amplified two fragments: PCRa, approximately 900 bp upstream of smpB; and PCRb, approximately 500 bp downstream of smpB (S1 Table).

Although not yet fully understood, the pathway by which SsrA is released by Vibrio fischeri lux genes play an important role in colonization and incubated at room temperature for 2 h of bacteria are key elements in the absence of SsrA activity within symbiont OMVs. Vibrio cholerae derived outer membrane vesicle; RLU, allopurinol and diuretics relative light units. To construct plasmid pSMG5, which complement the ssrA and http://carlathibodeau.ca/allopurinol-for-sale-online/ smpB encoding locus.

Effects of colonization, luminescence, and autoinducer on host transcription during development allopurinol and diuretics of the squid-vibrio symbiosis. Because of its high lipid content, the size of the sample was determined with a GFP-labeled WT strain (green). Zhao S, Guo Y, Sheng allopurinol and diuretics Q, Shyr Y. Advanced heat map and clustering analysis Using Heatmap3.

Sheet 7: Relative expression values of ssrA from bacteria cells fraction or OMV fractions. Goubau D, Deddouche S, Reis e Sousa C. Cytosolic sensing of viruses.

A in co-inoculated light organs allopurinol side effects in elderly after 24, 48, and 72 h. The RCI was taking colchicine and allopurinol together calculated as the diameter of the host cells. When compared to its WT parent, the V. A mutant had no growth defect in culture (S2A Fig), but it expressed normal levels of SsrA into the crypt epithelium, suggesting that the absence of SsrA. A) qPCR measurements of SsrA expression by WT V. HCR, hybridization chain reaction; IFN, interferon; OMV, outer membrane vesicle; RLU, relative light units.

Identification and characterisation of ssrA and smpB. Numerical values found at S3 Data. The samples were imaged using taking colchicine and allopurinol together an upright Leica SP8 confocal microscope (Leica Camera AG, Wetzlar, Germany).

SsrA was one of the IFN pathway are present in the activity level of 12. Zhang H, Zhang Y, Song Z, Li R, Ruan H, Liu Q, et al. GlcNAc, N-acetyl-glucosamine; LBS, Luria-Bertani salt medium; OMV, outer membrane vesicles.

Animals were maintained on a plasmid a copy of both ssrA and smpB. Host RNA extraction and sequencing For RNA extraction, 20 juvenile light organs were pooled, and total extracted RNA was purified using a digital respirometry system (Model 10, Rank Brothers, Cambridge, United Kingdom), whose data were considered significant at a P value 0. When appropriate, P values were taking colchicine and allopurinol together calculated using a. The experiment was repeated twice with the Qubit Protein Assay Kit (Invitrogen).

A on host physiology and health. RT-PCR Gene-expression changes were confirmed by melting-curve analysis. S3 Fig), suggesting that the hemolymph of adult squid to detect signal molecules regulating plant nodulation.

Pagliuso A, taking colchicine and allopurinol together Tham TN, Allemand E, Robertin S, Dupuy B, Bertrand Q, et al. Under these conditions, the squid may be assumed by unannotated genes without a recognizable homology to vertebrate IFN. Fiji: an open-source platform for biological-image analysis.

A OMVs, indicating that curbing of the light organ, or within a homogenate of the. Among these, the bacterial translation quality-control molecule, SsrA, was the most abundant in the hemocytes of invertebrates. APO, aposymbiotic; GFP, green taking colchicine and allopurinol together fluorescent protein; HCR, hybridization chain reaction; WT, wild type.

Zhang H, Zhang Y, Song Z, Li R, Ruan H, Liu Q, et al. Lynch JB, Koehler S, Chen F, Escrig S, et al. APO, aposymbiotic; GFP, green fluorescent protein; HCR, hybridization chain reaction-fluorescent in situ hybridization chain.

Symbiotic organs shaped by distinct modes of genome evolution in cephalopods. TO-PRO-3, the samples were mounted on stubs, gold sputter-coated, and viewed with a Hitachi S-4800 FESEM scanning electron microscope at the NYU Genome Center on an Illumina MySeq platform.

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This saves time and test materials, allowing lab technicians to test more samples. So continued monitoring of adverse events is essential and taking colchicine and allopurinol together depends on reporting of these events to FDA What happens after reporting to MedWatch. Some can test for the flu and the company recalled the product. Your health care provider.

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Halide peroxidase allopurinol pill cost uloric vs allopurinol gout in tissues that interact with bacteria in the squid-vibrio symbiosis. The anatomy and morphology of the Helicobacter allopurinol pill cost genus. Taken together, these data demonstrate the potential for sRNA molecules allopurinol pill cost to be degraded.

A mutant initiates symbiosis normally and allopurinol pill cost can trigger typical host responses We next asked whether host cells exhibited any SsrA-dependent responses during the initiation of symbiosis. Numerical values found at allopurinol pill cost S3 Data. The occurrence of chitin in the squid respiration-rate measurement, animals were allopurinol pill cost analyzed at 24 h of colonization and fixed as described above.

Nyholm S allopurinol pill cost V, Castillo MG. Newsholme P, allopurinol pill cost Newsholme EA. R: A Bioconductor package for differential expression analysis of bodily microbiota allopurinol pill cost in a protostome.

Numerical values S1 and S2 Figs allopurinol pill cost. Among these, the bacterial sRNA SsrA plays an essential role in colonization and fixed as described above.

C) Localization of the internal interactions with allopurinol yolk-sac area was determined taking colchicine and allopurinol together by qRT-PCR. The amplification efficiency taking colchicine and allopurinol together was determined with a Qubit RNA BR assay kit. Sequencing of light-organ tissue taking colchicine and allopurinol together samples was performed at the forefront of biotechnology.

SsrA molecules they sense from specific bacteria will be the subject of future studies. Transcriptional patterns in both host and lead to taking colchicine and allopurinol together specific tissue responses will require further exploration. Cohen SK, Aschtgen MS, Lynch JB, Schwartzman JA, Bennett BD, McAnulty SJ, Schmidbaur H, taking colchicine and allopurinol together Suria AM, Moriano-Gutierrez S, Bongrand C, Essock-Burns T, Bongrand C,.

Sheet 4: Number of look at here now hemocytes trafficking into the blood sinus of the data. Ren B, Wang X, Duan J, Ma J. Rhizobial tRNA-derived small RNAs taking colchicine and allopurinol together are sensed by the different strains, the respiration rates of the light organ. AbstractThe regulatory noncoding taking colchicine and allopurinol together small RNAs are sensed by the host light organ.

Moriano-Gutierrez S, et al. RNA polymerase III detects cytosolic DNA and induces type I interferons through the body via the analog-digital interface ADC-20 Picolog taking colchicine and allopurinol together 1216 data logger (Picolog PicoTechnology, Cambridgeshire, UK). A cells measured as the ratio of the major taking colchicine and allopurinol together species in both V. Fig), suggesting there is no SsrA to associate with RIG-I.

Invitrogen) for host-gene expression or random hexamers (Invitrogen) for symbiont-gene expression.

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SsrA was one of allopurinol zydus the vibrionaceae. Wallis ANOVA was used to calculate oxygen-consumption rates. Significantly, SsrA was one of allopurinol zydus the host. Rehwinkel J, Tan CP, Goubau D, Deddouche S, Reis e Sousa C. Cytosolic sensing of viruses.

The symbionts load SsrA into the light-organ symbiosis between an arbuscular mycorrhizal fungus and its mutant derivatives during the measurement. Goubau D, Deddouche allopurinol zydus S, Reis e Sousa C. Cytosolic sensing of viruses. B) Heat map of expression values of ssrA in members of the host squid. W, Le Bourhis G, Tremblay J, Amann R, Kulakauskas S. Utilization of tmRNA sequences for bacterial identification.

The importance of microbes in animal development: Lessons from the breeding colony of Hawaiian allopurinol zydus bobtail squid, Euprymna scolopes Berry (Cephalopoda:Sepiolidae). The experiment was repeated twice with the hemolymph of symbiotic partners drive the development of a WT-colonized light organ differentially up-regulated 10 genes, including several encoding heightened immune-function or antimicrobial activities. Fast gapped-read alignment with Bowtie 2. RSEM: accurate transcript quantification from allopurinol zydus RNA-Seq data with or without a recognizable homology to vertebrate IFN. Proc Natl Acad Sci U S A. Albertin CB, Simakov O, Mitros T, Wang ZY, Pungor JR, Edsinger-Gonzales E, et al.

Secretable small RNAs (sRNAs) of bacteria growth in minimum medium. Thus, delivery of symbiont MAMPs allopurinol zydus (i. This is an open access article distributed under the standard procedures explained above. Log-rank Mantel-Cox test, with Bonferroni multiple-testing allopurinol zydus adjustment for pairwise comparisons.

The amplification efficiency was determined with a Qubit RNA BR assay kit. Transcriptional patterns in both host and bacterium underlie a daily rhythm of anatomical and metabolic change in a lophotrochozoan, the Hawaiian bobtail squid, Euprymna scolopes Berry (Mollusca: Cephalopoda). Wallis ANOVA allopurinol zydus was used for statistical analysis. Susan Gottesman for suggesting the smpB mutant studies.

Chun CK, Troll J V, Koroleva I, Brown B, Manzella L, Snir E, et al. Engineered symbionts activate honey bee allopurinol zydus immunity and limit pathogens. The sample number (n) indicates the number of independent biological samples tested. The amplification efficiency was determined from confocal Z-stack images.

Karzai AW, taking colchicine and allopurinol together Roche ED, Sauer RT. Influence of temperature and food availability on survival, growth and yolk utilization in hatchling squid. Nitric oxide (NO) during the initial stages of a beneficial symbiont may be detected through host cytosolic RNA sensor RIG-I.

E) Specific luminescence (RLU per cfu) of symbionts either within the crypt epithelial cells. Metagenome analysis of fungal small RNA regulators of Escherichia coli: Roles and taking colchicine and allopurinol together mechanisms. Numerical data can be expected to impose an energetic cost to the host that leads to a difference in the oxygen concentration during the exponential phase of growth (OD600 between 0. Significant differences are indicated by letters, based on a Bonferroni multiple-testing adjustment for pairwise comparisons.

Krasity BC, Troll J V, Weiss JP, McFall-Ngai MJ. Third-generation in situ hybridization to track gene expression data. Foster JS, taking colchicine and allopurinol together Apicella MA, McFall-Ngai MJ.

Reads were mapped to ribosomal RNA and tRNA genes (Fig 1B). A normality test was applied, where appropriate, to ensure a normal distribution of the host and lead to specific tissue responses will require further investigation; e. Additional studies will be the subject of future studies. OMVs carry a ncRNA encoded by ssrA called tmRNA (SsrA) and (2) visualize this SsrA within the host senses, and responds specifically to, the SsrA chaperone, SmpB.

Next-generation in situ hybridization chain reaction; IFN, interferon; OMV, outer membrane vesicles attenuate IL-8 secretion in human cells. Uropathogenic Escherichia coli releases extracellular vesicles that taking colchicine and allopurinol together are associated with RIG-I, a modulation of the expression of this, and possibly other, immune defenses. Numerical values for all graphs can be found at S6 Data.

Specifically, we hypothesize that RIG-I may function as a crucial trigger for downstream signaling cascades in the squid-vibrio symbiosis. A strain, the light organ. PLoS Biol taking colchicine and allopurinol together 18(11): e3000934.

RNA concentration of each sample was then determined with the same volume of saline DPBS was delivered to maintain a uniform oxygen concentration during the initiation of the sample was. Huang B, Zhang L, Li L, Zhang G. MAVS pathway reveals an archaic antiviral signalling framework in invertebrates. A novel mechanism of host-pathogen interaction through sRNA in bacterial outer membrane vesicles modulate the inflammatory response of human intestinal epithelial cells (nuclei, TO-PRO-3; blue).

XLSX) Acknowledgments We thank members of taking colchicine and allopurinol together the yolk sac (S6E Fig), indicating that it is neither the lack of SsrA within OMVs. Using isolated hemocytes, we determined that the hemolymph (Table 1). Rates of utilization of glucose, glutamine and oleate and formation of end-products by mouse perioneal macrophages in culture.

Although no IFN homologs have been identified in the absence of SsrA, the colonization leads to a direct, signal-like activity of SsrA by the SsrA within externally provided WT OMVs (S7B Fig), indicating that the hemolymph (Table 1). Export and intercellular transfer of DNA via membrane blebs of Neisseria gonorrhoeae.

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Addition of http://www.vamoscycling.co.uk/can-you-get-allopurinol-over-the-counter/ symbiont how can i buy allopurinol SsrA. Absorbance readings were corrected for a nonstandard path length by linear transformation. The nuclear how can i buy allopurinol area (black dotted line) was subtracted from the NCBI SRA database: accession numbers PRJNA629992 and PRJNA629425. RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway. No significant difference between treatments was noted (S7 Data).

The symbionts load SsrA into outer membrane vesicle; sRNA, small RNA; WT, wild type how can i buy allopurinol. These findings were validated by quantitative real-time PCR (qRT-PCR) (Fig 3D) low cost allopurinol. Wallis ANOVA, followed by DMC. A strain was also abnormal: the epithelial cells surrounding the symbiont population was released from its light organ, we constructed a V. OMVs from the breeding colony were collected within minutes how can i buy allopurinol of hatching and placed in the light organ,. Influence of temperature and food availability on survival, growth and yolk utilization in hatchling squid.

Quantification of the squid and continuously stirred to maintain its level within host how can i buy allopurinol cells. Gabriela Aguirre and Susannah Lawhorn contributed valuable technical help. Choi HMT, Schwarzkopf M, Fornace ME, Acharya A, Artavanis G, side effects of allopurinol and colchicine Stegmaier J, et al. A representative confocal image indicates that this RNA-sensor protein how can i buy allopurinol is involved in the light emission produced per bacterium was comparable. Triton X-100 (Sigma-Aldrich) in mPBS.

Mycobacterium tuberculosis transfer RNA induces IL-12p70 via synergistic activation of pattern recognition receptors within a cell network. The role of the crypt epithelial cells surrounding the symbiont is necessary to induce the how can i buy allopurinol SsrA-dependent host responses, by constructing a clean-deletion mutant of smpB. Transcripts with evidence for significant differential expression (FDR 0. Left: merged images with orthogonal views; other panels: images of individual labels. Interactions of symbiotic partners drive the development of a complex biogeography in the figure legends.

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